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Instructions, Notes and Protocols for LightCycler 480 Real Time PCR Users

 
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Notes

Methods and protocols

Roche Real-Time Worksheet.xls

Manuals

LC480 Operator's Manual.pdf
LC480 Relative Quantification.pdf
LC Primer Design Software.pdf

Technical Notes - Roche

Selected references on Real-Time PCR

General:

  • Bustin SA, Nolan T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J Biomol Tech. 2004 Sep;15(3):155-66. Review. PMID: 15331581. Available at http://jbt.abrf.org

    Housekeeping and reference genes:

  • Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible WR. Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol. 2005 Sep;139(1):5-17. PMID: 16166256. Available at http://www.plantphysiol.org

  • Dheda K, Huggett JF, Chang JS, Kim LU, Bustin SA, Johnson MA, Rook GA, Zumla A. The implications of using an inappropriate reference gene for real-time reverse transcription PCR data normalization. Anal Biochem. 2005 Sep 1;344(1):141-3. PMID: 16054107. Available at http://www.sciencedirect.org

  • Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun. 2004 Jan 23;313(4):856-62. PMID: 14706621. Available at http://www.sciencedirect.com

     

     

     

     

     

     

     

     


  • This site was last updated by Vijaya Rao on March 27, 2009.